SCIENTIFIC POSTERS DOWNLOAD
Here are the 2 scientific posters featured during the last IEIDC in Deauville.
If you have not been able to attend it, you can still download the posters to understand how LAMP-PCR is a care tool to help diagnose equine diseases.
Authors: L. Valot, T. Thibault, M. Simonnet, D. Cormier, A. Lacaze, V. Luong, D. Schieb and L. Thiery
Abstract
LAMP technology (Loop mediated isothermal AMPlification) is a NAAT technology (Nucleic Acid Amplification test) which can occur at constant temperature, unlike PCR which need thermal cycles. This allow the use of this technology as a POC (point of care) test and allow the obtention of the result in only 30 min without the necessity to use a thermal cycler. This technology can be really useful to detect highly contagious pathogens causing diseases in animals and the possibility to administrate medicine or isolate the animal immediately. In case of a pandemic, this technology can be useful to isolate quickly contaminated animals and avoid a propagation of the pathogen. Specific LAMP primers for EHV-1/EHV-4/Equine Influenza/Streptococcus equi equi were designed and LAMP reactions were performed on pathogen genome and on nasopharyngeal swab samples extracted by our own specific quick extraction. Performances of these tests were evaluated in our lab and compared to PCR performed in external labs. LAMP tests have a limit of detection for amplification between 1-40 copies/reaction dependant of the test. The limit of detection of the method (quick extraction and LAMP test) is similar or better of the limit of detection of the method (extraction on column and PCR test) obtained in a conventional analysis laboratory. LAMP test which was designed are really specific for the target (>95% specificity) and are really sensitive for the target (>90% sensibility). This technology is really fast (<30 min) and can be used as a point of care test. LAMP tests are a new generation of DNA/RNA amplification test, really specific, really sensitive which can be performed near a sick animal and can give a result in only 30 min. These parameters allow the consideration to use this technology to analyse quickly potential pathogens holder and treat or isolate the animal immediately.
Authors: A. Zocevic, T. Thibault, L. Valot, M. Simonnet, L. Melo, D. Cormier, A. Lacaze, V. Luong, D. Schieb and L. Thiery
Abstract
LAMP technology (Loop mediated isothermal AMPlification) is a NAAT technology (Nucleic Acid Amplification test) which can occur at constant temperature, unlike PCR which need thermal cycles. This allow the use of this technology as a POC (point of care) test and allow the obtention of the result in only 30 min without the necessity to use a thermal cycler. This technology can be really useful to detect highly contagious pathogens causing diseases in animals and the possibility to administrate medicine or isolate the animal immediately. Specific LAMP primers for Theileria equi, Babesia caballi, Anaplasma phagocytophilum, were designed and LAMP reactions were performed on the genome of the pathogen and on blood samples extracted by our own specific quick extraction. Performances of these tests were evaluated in our lab and compared to PCR performed in external labs. LAMP test which was designed are really specific for the target (>93% specificity) and are really sensitive for the target (>90% sensibility). This technology is really fast (<40 min) and can be performed near a sick animal. In this case, it can help a veterinarian to determine if the use of imidocarbe or antibiotic could be useful to treat the animal. LAMP tests are a new generation of DNA/RNA amplification test, really specific, really sensitive which can be performed near a sick animal and can give a result in only 40 min. These parameters allow the consideration to use this technology to analyse quickly potential pathogens holder and treat or isolate the animal immediately.
