3 ASSETS PROVIDED BY THE LAMP
- Speed: no temperature cycling
- Easy-to-use: no need for DNA purification, few steps for extraction
- Robustness: no resistance to PCR inhibitors
ANIMAL HEALTH - ready-to-use diagnostic kits
Enalees offers you a simple, fast and innovative analysis for the diagnosis of many infectious and respiratory diseases (whatever their degree of progression) in horses, dogs and cats.
DNA AMPLIFICATION METHOD USED: LAMP
Enalees tests use a technology called LAMP (Loop-mediated isothermal amplification). Unlike PCR (Polymerase Chain Reaction), this technology allows the amplification of the pathogen genome at constant temperature thanks to a strand displacement DNA polymerase which does not require temperature cycling to dehybridize the DNA.
The advantages of LAMP are:
- its flexibility: no temperature cycle
- its simplicity: no need for DNA purification, few steps for extraction, multiple primers spanning across several hundred basepairs ensure a higher specificity
- its robustness: more resistance to PCR inhibitors
PRESENTATION OF THE ENALEES TESTS
The Enalees tests are presented in individual single-use pouches containing all the reagents and consumables necessary to perform a test.
Depending on the reference, each test can detect from one to five pathogens and is validated by a positive control. This positive control confirms the correct sampling as well as the correct functioning of the test and the analyser.
All handling steps are explained on the rack provided in the box and also on the quick user guide. Just follow the steps and it will only take a few minutes.
WHEN TO USE AN ENALEES TEST?
When clinical signs such as hyperthermia are present in an animal, this implies that following an infection, pathogens are present in one or more biological samples (blood, urine, etc.). As the immune response is not immediately detectable, serological tests cannot be used at the beginning of an infection and only molecular biology tests, such as the Enalees Test, are effective in identifying the disease responsible for these symptoms.
Usually, after one to two weeks, and if the animal survives the infection, different types of antibodies appear, making the use of serological tests possible. However, proof of infection can only be provided by performing two serological tests within a given interval to demonstrate seroconversion. Under these conditions, molecular tests are still relevant.
Enalees tests can be used from the early stages of infection as they directly detect the DNA or RNA of the pathogen.
ENALEES PRODUCT RANGES
EPONA
Epona tests are designed to detect equine infectious diseases. They allow the detection of pathogens responsible for “piro-like” syndromes, otherwise known as isolated fevers, as well as pathogens responsible for acute respiratory syndrome.
ASTÉRIA
The Asteria tests, intended for dogs and cats, allow the detection of enteropathic diseases as well as coryza.