CANINE TICK DISEASES EHRLICHIOSIS: THE DIAGNOSIS OFTEN FORGOTTEN
CANINE TICK DISEASE
Many pathogens are the cause of tick-borne diseases in dogs, such as Babesia canis, Babesia gibsoni, Anaplasma platys, Ehrlichia canis, among others. These pathogens are carried by ticks belonging to the genera Dermacentor, Ixodes and Rhipicephalus, which transmit them to the dog during their bite, via their saliva.
Dogs infected with a tick-borne disease may exhibit a variety of clinical symptoms, such
as hyperthermia, lethargy, loss of appetite, dark urine, pale or yellowish mucous membranes, and occasionally vomiting.
EHRLICHIOSIS
In the acute phase of Ehrlichiosis (also called Anaplasmosis) caused by Anaplasma platys, Anaplasma phagocytophilum or Ehrlichia canis, the clinical signs may go unnoticed. If nothing is done, pathogens continue to multiply in the dog’s blood. Some of them have a high tolerance to the bacterial load while other breeds, such as the German Shepherd, can develop a chronic form of the disease. It should be noted that, during stress, for example, the disease can occur even though the bite dates back a few months.
During a canine Ehrlichiosis infection, co-infection can take place in 50% of cases with other pathogens transmitted by the same vector. The chronic form of Ehrlichiosis is very serious and can cause the dog to lose weight, joint pain and bleeding.
At this stage, the bone marrow is irreversibly affected. Blood cells are no longer produced in sufficient quantities and the chances of survival of the animal are very low.
STANDARD DIAGNOSTIC PROTOCOL
During the acute phase of infection, tick-borne diseases can be diagnosed by various methods:
- Serological method
Perform tests 2 weeks apart to verify Seroconversion- By indirect immunofluorescence
- By fixing the complement
- By ELISA techniques (mainly used for chronic piroplasmosis)
- Biological or biochemical method
- By CBC: anaemia and thrombocytopenia
- By biochemistry: hyperglobulinemia
- Research method
the pathogen or its genome- By blood smear (low sensitivity)
- By PCR (to be done before antibiotic treatment)
FOR OPTIMAL EFFICIENCY, it is recommended to use several of these methods, as they detect different elements and complement each other.
PREVENTION OF TICK-BORNE DISEASES IN DOGS: PRACTICAL RECOMMENDATIONS FOR VETERINARIANS
Tick-borne diseases, such as babesiosis and ehrlichiosis, represent a constant challenge to canine health. Despite the lack of vaccines for some of these diseases, there are effective preventive strategies that veterinarians can recommend to their clients
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- Regular use of parasite control treatments:
Parasite control treatments remain essential in the prevention of tick infestations. Recommend the use of acaricidal collars, pipettes, sprays or tablets, tailored to each dog’s specific needs and environment.
- Where to check your dog for ticks:

- Regular use of parasite control treatments:
Parasite control treatments remain essential in the prevention of tick infestations. Recommend the use of acaricidal collars, pipettes, sprays or tablets, tailored to each dog’s specific needs and environment.

- Reducing exposure to ticks:
To limit the risk of infestation, advise avoiding walks in tall grass or in the forest, especially during periods of high tick activity.
These areas represent
an increased risk of contamination for dogs.
By combining these preventive measures, veterinarians can help protect effectively dogs against tick-borne diseases.
Continued owner education and a proactive approach remain key to reducing the incidence of these diseases.
DIAGNOSTIC
COMPARISON OF THE DIFFERENT DIAGNOSTIC METHODS ON ACUTE CASES
Acute tick-borne diseases in dogs, such as babesiosis and ehrlichiosis, require a quick and accurate diagnosis for effective management.
Several methods are available to identify the pathogens involved. Light microscopy allows the identification of ticks, the examination of pathogens in their salivary glands, or in the biological samples of the infected animals while serology detects the presence of specific antibodies directed against the pathogen infecting the animal (it is therefore an indirect detection of the pathogen). PCR, on the other hand, offers sensitive and direct detection of pathogens via their DNA, even in small quantities.
The combination of these methods improves the reliability of the diagnosis.

OUR ENALEES SOLUTION
Advantages of PCR-LAMP tests over serological tests in the aid of diagnosis:
Our Asteria® tests are molecular biology tests. They directly detect the DNA of the pathogen and can therefore be used from the beginning of the infection even before symptoms are detectable or the immune response is put in place.
Their sensitivity is very close to that of PCR, but the results are obtained in 30 minutes.
