Here are the 3 scientific posters featured during the last ISWALVD in Lyon.
If you have not been able to attend it, you can still download the posters to understand how Enalees can support you in your diagnostic journey either as a veterinary professional or as a diagnostic development & manufacturing manager.
Authors:: Aleksandar Zocevic, Thomas Thibault, Laurine Valot, Mégane Simonnet, Luana Melo, Dove Cormier, Alban Lacaze, Viviane Luong, Delphine Schieb & Laurent Thiery
The piro-like syndrome in equines refers to a complex set of often numerous and non-specific clinical signs such as hyperthermia, depression, anorexia, anemia or jaundice. Theileriosis, Babesiosis and Anaplasmosis are known to be correlated to this syndrome and can be caused by parasites called Theileria equi, Babesia caballi as well as pathogenic bacteria Anaplasma phagocytophilum, respectively.
The Epona Theileria Babesia Anaplasma tests are based on the LAMP technology (1). They allow detection in blood of a genomic fragment of pathogenic T. equi, B. caballi, and A. phagocytophilum in a reliable and rapid manner to obtain indications as to the presence or absence of the pathogen(s) incriminated in the appearance of the clinical signs observed. Then, the most appropriate treatment can be early provided to the horse as Theileriosis, Babesiosis and Anaplasmosis require different drugs.
(1) See poster P-33 for description of LAMP technology.
Unlike traditional PCR reactions, Enalees tests are performed in few steps on a portable and easy-to-use reader system. The result can therefore be obtained in less than 30 minutes directly in your clinic (2). Performances of these tests were compared to PCR performed in external labs and results are revealed below.
(2) Enalees also provides Asteria tests for detection of pathogens in cats and dogs. Check out our website for further information.
Authors: Thomas Thibault, Laurine Valot , Mégane Simonnet, Aleksandar Zocevic, Walid Yakoub, Luana Melo, Méryne Gêne, Delphine Schieb, Esteban Schaeffer & Laurent Thiery
Currently, the direct detection of pathogens contaminating animals can be performed by
LAMP technology (Loop mediated isothermal AMPlification) is a NAAT technology (Nucleic Acid Amplification test) which can occur at constant temperature, unlike PCR which need thermal cycles. This allow the use of this technology as a POC (point-of-care) test and allow the obtention of the result in only 30 min without the necessity to use a thermal cycler. This technology can be really useful to detect highly contagious pathogens causing diseases in animals and the possibility to administrate medicine or isolate the animal immediately.
Authors: Laurine Valot , Thomas Thibault, Mégane Simonnet, Dove Cormier, Alban Lacaze, Viviane Luong, Delphine Schieb, Laurent Thiery
Respiratory diseases are common in horses and can spread really fast with horse meetings and sport events. This spreading can lead to serious epizootic disease (as seen in France in 2022 and 2023) with economical consequences and grave symptoms/consequences for contaminated horses. To prevent the spreading of these pathogens, infected horses have to be tested as soon as possible, to be isolated from the others, and to receive an adapted treatment.
Among pathogens responsible for respiratory diseases, the most commons and serious are Equine Herpes Virus 1 (EHV-1), Equine Herpes Virus 4 (EHV-4), Equine Influenza Virus (EIV) and streptococcus equi subs. equi (responsible of strangle). For herpes viruses and influenza virus, only a symptomatic treatment is used, whereas for strangle the administration of antibiotics is recommended. As symptoms of these diseases could be the same and quite unspecific such as respiratory troubles, cough, nasal discharge and fever, it is important to differentiate which pathogen is infecting the horse.
Commonly, veterinary professionals identify which pathogen is present on a nasopharyngeal swab by PCR assay. However, they have to send the sample to an external laboratory and wait a few days before receiving the result. This process delays the diagnosis, thus treatment and quarantine. By using a LAMP assay (Loop mediated isothermal AMPlification) with a quick extraction, they can discriminate the pathogen in less than 30 minutes and facilitate their work.